| Registro completo |
| Provedor de dados: |
Acta Amazonica
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| País: |
Brazil
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| Título: |
A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment
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| Autores: |
MANGARAVITE,Érica
TERRA,Vanessa
HATTORI,Eric Koiti Okiyama
DAL’SASSO,Thaís Carolina da Silva
BHERING,Leonardo Lopes
OLIVEIRA,Luiz Orlando de
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| Data: |
2020-12-01
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| Ano: |
2020
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| Palavras-chave: |
Ascorbic acid
Dithiothreitol
DNA isolation
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| Resumo: |
ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.
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| Tipo: |
Info:eu-repo/semantics/report
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335
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| Editor: |
Instituto Nacional de Pesquisas da Amazônia
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| Relação: |
10.1590/1809-4392202001571
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| Formato: |
text/html
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| Fonte: |
Acta Amazonica v.50 n.4 2020
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| Direitos: |
info:eu-repo/semantics/openAccess
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